Microfluidic system for applying shear flow to endothelial cells on culture insert with collagen vitrigel membrane

This study proposes a microfluidic system comprising channel and cylindrical hole for holding commercially available culture insert with collagen vitrigel membrane. Because the bottom surface of top are in same plane, flow medium can be directly applied to cells on surface. Additionally, is easily removable from various biological analyses standard protocol, making this accessible users who not experts engineering. To demonstrate potential applications system, we confirmed that by applying medium, endothelial cultured myotubes aligned depending direction. We believe will useful tool morphological based fluidic shear stress. • The allows application stress morphology membrane was controlled system. Confocal microscopy TEER measurements were performed